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Get the complement, reverse complement, or reverse of a DNA sequence, or transcribe DNA to RNA, with base counts and GC content. FASTA and IUPAC codes supported.
Also covers: Reverse complement ยท DNA to RNA converter ยท Complementary DNA strand ยท GC content calculator
The other strand, read 5โฒ โ 3โฒ.
Paste a sequence to see the result.
Lowercase stays lowercase (often used for masked regions). IUPAC ambiguity codes are complemented too: RโY, KโM, BโV, DโH; S, W and N stay the same.
Plain bases or FASTA; header lines, spaces, line numbers, and gaps are ignored. You can also drop a .fasta or .txt file.
Reverse complement, complement, reverse, DNA โ RNA from the coding strand, RNA from a template strand, or RNA back to DNA.
The output is wrapped at 60 bases per line, with the sequence length, base counts, and GC content.
The complement pairs each base (AโT, CโG) in the same order, so it reads 3โฒ โ 5โฒ. The reverse complement also reverses it, giving the other strand in the standard 5โฒ โ 3โฒ direction, which is what primers and databases use.
If your sequence is the coding (sense) strand, the mRNA has the same sequence with U instead of T: use DNA โ RNA. If it's the template (antisense) strand, use RNA from template, which takes the reverse complement and writes U for T.
A, C, G, T and U, plus the IUPAC ambiguity codes (N, R, Y, K, M, S, W, B, D, H, V), which are complemented too. Lowercase letters stay lowercase.
No. The sequence never leaves your browser.
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